Fig. 4

To putatively identify the remaining lichen RiPP BGCs (those not in BiGSCAPE clans) with their closest known homologous cluster, we examined the conserved domains in each RiPP signature gene detected by AntiSMASH. Based on the presence or absence of these domains, we classified the lichen RiPPs into putative ustiloxin, asperipin-2a, and phomopsin homologous gene clusters. It is important to note that this classification is based solely on the conserved domains, not on cluster homology or synteny, meaning these clusters are the closest known homologs but remain distinct from the ustiloxin, asperipin-2a, and phomopsin clusters. This figure shows the distribution of the three signature domains, or their absence, across various lichens. The absence of the domain is indicated by empty circles, presence by colored circles. The three shades indicate three different putative dikaritin gene clusters identified by MIBiG integrated within AntiSMASH (similarity ranging from 0.1 to 0.3). Dark brown circles represents putative ustiloxin homologs, red ones indicates asperipin-2a, and pink ones denotes phomopsin homologous gene clusters. These clusters are distinct from those that cluster within R1 and R2 clans. The black dots indicate bootstrap values > 70.