Fungal Biology and Biotechnology

Table 3 Selection conditions applied in SELEX-3

From: Development of a whole-cell SELEX process to select species-specific aptamers against Aspergillus niger

Whole-cell SELEX-3
Round	ssDNA		Positive selection		Reaction volume (µL)	Competitors	Washes	Counter-selection
	pmol	Final conc (nM)	Target strains	Number of conidia
1	1800	7200	ATCC 1015, CBS 554.65	10⁷	250	–	1x	–
2	25	100	ATCC 1015, CBS 554.65	10⁷	250	BSA, 4sDNA	1x	–
3	23	92	ATCC 1015, CBS 554.65	10⁷	250	BSA, 4sDNA	2x	–
4	23	92	ATCC 1015, CBS 554.65	10⁷	250	BSA, 4sDNA	2x	A. tubingensis MA 3973
5	9.2	92	ATCC 1015, CBS 554.65	10⁷	100	BSA, 4sDNA	3x	A. tubingensis MA 3973
6*	9.2	92	ATCC 1015, CBS 554.65	10⁷	100	BSA, 4sDNA	3x	A. tubingensis MA 3973
7	9.2	92	ATCC 1015, CBS 554.65	10⁷	100	BSA, 4sDNA	3x	A. tubingensis MA 3973
8	9.2	92	ATCC 1015, CBS 554.65	10⁷	100	BSA, 4sDNA	5x	A. tubingensis MA 3973
9	9.2	92	ATCC 1015, CBS 554.65	10⁷	100	BSA, 4sDNA	5x	A. tubingensis MA 3973
9-N	9.2	92	ATCC 1015, CBS 554.65	10⁷	100	BSA, 4sDNA	5x	–
9-T	9.2	92	A. tubingensis MA 3973	10⁷	100	BSA, 4sDNA	5x	–

*ssDNA applied to round 6 was derived from a mixture of dsDNA obtained from the amplification of the ssDNA recovered after round 5 and amplified from the already amplified ssDNA

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