Fig. 3
From: Genomic deletions in Aureobasidium pullulans by an AMA1 plasmid for gRNA and CRISPR/Cas9 expression
Phenotype of selected transformants. Growth of the wild-type and obtained transformants was tested on plates with the indicated medium. From the first transformation assay (row 1) the transformant 1.2 (∆URA) and the WT are displayed. From the second transformation (row 2) the 33 selected transformants were tested and are displayed together with the wild-type (WT), the URA3 mutant strain #6 from [19] (#6), and an A. pullulans EXF-150 double deletion mutant with a hygromycin B genomic resistance cassette (∆∆). Tran number of transformations, MEX malt extract, uri uridine, hyg hygromycin B, 5-FOA 5-fluoroorotic acid